Stage 1 TGCTs Expression Profile

Gene Expression Signatures Prognostic for Relapse in Stage I Testicular Germ Cell Tumors (TGCT)

Description: Design, Setting and Participants We reviewed patients with SI non-seminoma (NS) and seminoma (S) from an institutional database (2000-2012) managed with active surveillance. NR-NS and NR-S patients were defined as having no evidence of relapse after 2 and 3 years of surveillance respectively. RNA extraction and gene expression analysis was performed on archival primary tumor samples. Outcome measurements and statistical analysis Gene-Set-Enrichment-Analysis (GSEA) was conducted in order to identify discriminating biological pathways associated with differentiation of R from NR and S from NS. Hierarchical clustering analysis and Wilcoxon testing were used to evaluate candidate genes and expression patterns that could differentiate NR from R. Results 57 patients (12 R-NS, 15 RS, 15 NR-NS, 15 NR-S) were identified with median relapse time of 5.6 (2.5-18.1) and 19.3 (4.7-65.3) months in NS and S cohorts respectively. 1039 differential expressed genes were identified that separated R and NR. In R patients, GSEA revealed enrichment in pathways associated with differentiation such as skeletal development (i.e. FGFR1, BMP4, GLI2, SPARC, COL2A1), tissue (i.e. BMP4, SPARC, COL13A1) and bone remodelling (i.e. CARTPT, GLI2, MGP). A discriminative gene expression profile between R and NR cases was discovered when combining NS and S samples using 10 (p=0.03) and 30 (p=0.03) probe signatures. However, this profile was not observed in the S and NS cohorts individually. Conclusions A discriminating signature for R and NR was identified for SI TGCT that was not histology specific. Genes associated with active differentiation were enriched in patients with R disease. Further validation is required to determine if this signature provides independent prognostic information to standard pathological risk factors.
Lewin J, Soltan Ghoraie L, Bedard PL, Hamilton RJ, Chung P, Moore M, Jewett MAS, Anson-Cartwright L, Virtanen C, Winegarden N, Tsao J, Warde P, Sweet J, Haibe-Kains B, Hansen AR
Lab: Haibe-Kains
Year: 2018


Lewin J, Soltan Ghoraie L, Bedard PL, Hamilton RJ et al. Gene expression signatures prognostic for relapse in stage I testicular germ cell tumours. BJU Int 2018 Nov;122(5):814-822. PMID: 29726090

60 samples

Sample Type:N/A


This dataset is public.

Contact: Benjamin Haibe-Kains

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